Innovating Science® - APï®® Chemistry Lab #5: Separation of Molecules

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Chemical products can only be purchased by Corporations and Educational Institutions. Private Individuals are not eligible.

Manufacturer ID - IS8105

Students will learn about acids and bases through a class discussion and then test a dilute acid and dilute base with neutral litmus paper to learn how the paper indicates something is acidic or basic. Once that skill is learned they can move on to test some common items that would be found around their house to determine if they are acids or bases. Optional: Extra litmus paper is included to test more items either from home or around the school building for a greater understanding of acids and bases. The activity contains enough materials for 6 groups of students.

Safety Data Sheet

Students collect data using different solvents to identify the optimal solvent for separation. They will then illustrate the intermolecular forces that are acting on the molecules in the separation. Students evaluate the chromatograph with different solvents and establish a connection between molecular structure and intermolecular attraction to the solvent. The activity contains enough materials for 15 groups of students as well as a Teacher's Guide and Student Study Guide Copymasters. Meets Big Idea 2, Investigation 5, Primary Learning Objective 2.10.

Kit Includes:
30mL Food Dye Blue #1, 0.5%
30mL Food Dye Yellow #5, 0.5%
30mL Food Dye Red #40, 0.5%
200mL 9:1 Petroleum Ether/Acetone
200mL Ethyl Alcohol, 95%
200mL Isopropyl Alcohol, 99%
200mL Acetone
200mL Deionized Water
15 Chromatography Paper
30 Glass Vials
4 Capillary Tubes

Materials needed but not supplied:
Metric Rulers

DOT Info:
UN1170, Ethanol, 3, II, Ltd Qty
UN1219, Isopropanol, 3, II, Ltd Qty
UN1993, Flammable liquid, n.o.s., (Petroleum ether, Acetone), 3, II, Ltd Qty
UN1090, Acetone, 3, II, Ltd Qty

WARNING: This product can expose you to chemicals including Methanol, which is known to the State of California to cause reproductive harm. For more information go to
Brand Name
Innovating Science®
Performance Expectations 5-PS1-3
Cross Cutting Concepts Cause and Effect
Scale, Proportion, and Quantity
Engineering Practices Planning and Carrying Out Investigations
Box Used 12663SBOX
Width (in) 6.25
Height (in) 4
Weight (oz) 19.9
Weight (lb) 1.24
Country of Origin US
Harmonization Code 9023.00.0000
DOT Description Non regulated

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