Innovating Science® - Thin Layer Chromatography
Manufacturer ID - IS2504
Students will learn about acids and bases through a class discussion and then test a dilute acid and dilute base with neutral litmus paper to learn how the paper indicates something is acidic or basic. Once that skill is learned they can move on to test some common items that would be found around their house to determine if they are acids or bases. Optional: Extra litmus paper is included to test more items either from home or around the school building for a greater understanding of acids and bases. The activity contains enough materials for 6 groups of students.
Product # IS8017
Chromatography is an analytical tool used to separate similar compounds. Thin-layer chromatography utilizes a sheet coated with silica gel. The samples are applied to the silica gel sheet and separated by migrating an appropriate solvent up the sheet. This type of separation is called adsorption chromatography because the separation of similar compounds is based on the selective adsorption of the compounds on the silica gel solid phase.
In this lab samples of various plant leaf materials may be extracted or the red and green leaf extracts included may be used. The samples of extracted leaf pigments are applied to a silica gel sheet and separated using a mixture of organic solvents.
2 x 10g Alfalfa Powder
2 x 25mL Red Extract
1 pkg of 15 Chromatography TLC Sheets
6 x 250mL Chromatographic Solvent
2 x 50mL Isopropyl Alcohol, 70%
UN1230, Methanol, 3, II, Ltd Qty
UN1219, Isopropanol, 3, II, Ltd Qty
WARNING: This product can expose you to chemicals including Methanol, which is known to the State of California to cause reproductive harm. For more information go to http://www.P65Warnings.ca.gov.
|Cross Cutting Concepts
|Cause and Effect
Scale, Proportion, and Quantity
|Planning and Carrying Out Investigations
|Country of Origin
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