Innovating Science® - AP Biology #8 Bacterial Transformation Kit

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Chemical products can only be purchased by Corporations and Educational Institutions. Private Individuals are not eligible.

Manufacturer ID - IS3708

Students will learn about acids and bases through a class discussion and then test a dilute acid and dilute base with neutral litmus paper to learn how the paper indicates something is acidic or basic. Once that skill is learned they can move on to test some common items that would be found around their house to determine if they are acids or bases. Optional: Extra litmus paper is included to test more items either from home or around the school building for a greater understanding of acids and bases. The activity contains enough materials for 6 groups of students.

Safety Data Sheet

Students will genetically engineer bacteria through transformation with a plasmid that confers antibiotic resistance. They will then examine the number of resistant bacteria to determine the efficiency of the transformation procedure. Teachers manual and Student Study Guide copymasters are included. Materials provided for 8 lab groups. Meets AP Science Practices 1, 3, 5, 6, and 7, and Big Idea 3.

Kit Includes:
30mL Sterile Calcium Chloride 0.05M
16 Sterile Volumetric Pipet
8 Pipet Bulb
8 Micropipet w/Plunger 10ul
1 Plastic Centrifuge Tube
16 Microcentrifuge Tube, 1.5mL
8 Sterile Inoculating Loop
40 Petri Dish
4 Luria Agar, 200mL
1 E. Coli Culture (freeze dried)
1 Luria Broth 9mL Tube

Kit Contains coupon for perishable materials. Redeem by fax, phone or e-mail.
Materials sent upon redemption of coupon:
2 btls 0.02g Ampicillin 1000 ng
1 pUC19 Plasmid
1 Tryptic Soy Broth Tube 9mL
1 Tryptic Soy Agar Slants 6mL
1 Pipet, Sterile, Disposable
1 pkg Cotton Swabs, Sterile

Materials Needed but not Supplied:
Large beaker Hot plate or waterbath
Microwave (not necessary if melting agar in a waterbath) Markers
Microfuge tube racks Container of ice

Recommended: Incubator (37®C)

DOT Info:
Brand Name
Innovating Science®
Performance Expectations 5-PS1-3
Cross Cutting Concepts Cause and Effect
Scale, Proportion, and Quantity
Engineering Practices Planning and Carrying Out Investigations
Box Used 12663SBOX
Width (in) 6.25
Height (in) 4
Weight (oz) 19.9
Weight (lb) 1.24
Country of Origin US
Harmonization Code 9023.00.0000
DOT Description Non regulated

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