Innovating Science® - Electrophoresis and DNA Fragment Length Determination

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Chemical products can only be purchased by Corporations and Educational Institutions. Private Individuals are not eligible.

Manufacturer ID - IS3300

Students will learn about acids and bases through a class discussion and then test a dilute acid and dilute base with neutral litmus paper to learn how the paper indicates something is acidic or basic. Once that skill is learned they can move on to test some common items that would be found around their house to determine if they are acids or bases. Optional: Extra litmus paper is included to test more items either from home or around the school building for a greater understanding of acids and bases. The activity contains enough materials for 6 groups of students.

Safety Data Sheet

Restriction enzymes are enzymes that are capable to cleaving, or cutting, DNA molecules in very specific spots based on the DNA molecule's sequence of bases. Their discovery and isolation in the latter half of the 20th century provided scientists with an invaluable tool and rapidly expanded in the field of molecular biology. Agarose electrophoresis is a technique for separating the pieces of DNA that result from the use of restriction enzymes. In this activity students will perform agarose electrophoresis on three DNA samples that have already been treated with restriction enzymes. One sample has been cut with the enzyme HindIII, one has been cut with the enzyme EcoRI, and one has been cut with both enzymes. The resulting pattern of DNA bands on the agarose gel will show that each enzyme affects the DNA differently. Students will not only learn about the process of DNA electrophoresis but learn the techniques associated with the process, such as setting up an agarose gel, loading DNA samples in the agarose gel, and staining the gel in order to visualize the DNA bands. They will also learn how to determine the sizes of unknown DNA fragments after examining their results.

The kit includes specially-treated DNA samples that do not require refrigeration or freezing, prepared agarose that may simply be melted in a hot water bath or microwave, TBE electrophoresis buffer, and DNA stain. There is enough DNA to run 10 gels.

Kit Contains:
200mL Prepared Agarose, 0.8% Solution
500mL Tris Borate ETA Buffer, 5X
60mL DNA Stain, 20X
100 ®L Lambda DNA/EcoRI Digest with Loading Dye
100 ®L Lambda DNA/HindIII Digest with Loading Dye
100 ®L Lambda DNA/EcoRI,HindIII Digest with Loading Dye

Needed but not supplied are agarose electrophoresis chambers, power supplies, and micropipettes capable of measuring 10®L.

DOT Info:
Brand Name
Innovating Science®
Performance Expectations 5-PS1-3
Cross Cutting Concepts Cause and Effect
Scale, Proportion, and Quantity
Engineering Practices Planning and Carrying Out Investigations
Box Used 12663SBOX
Width (in) 6.25
Height (in) 4
Weight (oz) 19.9
Weight (lb) 1.24
Country of Origin US
Harmonization Code 9023.00.0000
DOT Description Non regulated

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